Thursday, February 18, 2016

Spring 2016 First DNA Extraction

This week in lab I performed my first spider web DNA extraction and my second extraction ever!

Whenever you try something new you learn a few things. While doing extractions this week I learned: I should make sure my microcentrifuge tubes are free of DNA-degrading enzymes called DNases, there's a big difference between 1.5 mL and 2.0 mL when working with the tiny volumes used in DNA extractions, and that to keep DNases from being activated, the DNA must be on ice nearly constantly.

I am unsure if my extraction went well enough for me to prove I have DNA from webs just yet but I'll get it soon!

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